Device

Part:BBa_K392041

Designed by: Takuro A. Kagaya, Yuki Miyatake, Hirayama Ikumi   Group: iGEM10_Osaka   (2010-10-25)

exoglucanase secretion testing construct for E. coli

Device containing C. fermi exoglucanase Cex with E. coli PelB export tag attached upstream to enable secretion of protein.

See experience page for experimental data.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 821
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 280
    Illegal NgoMIV site found at 454
    Illegal NgoMIV site found at 827
    Illegal NgoMIV site found at 1329
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 874
    Illegal SapI.rc site found at 957


[edit]
Categories
Parameters
n/aexoglucanase secretion testing construct for E. coli